G0282

Sigma

 

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

from mouse, recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested

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Description

Analysis NoteThe EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.
Biochem/physiol ActionsGranulocyte-macrophage colony stimulating factor is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Physical formLyophilized from 10 mM acetic acid plus 250 μg BSA.

Properties

biological source from mouse
recombinantexpressed in Escherichia coli
assay≥97% (SDS-PAGE)
formlyophilized powder
potency≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)
mol wtmol wt 14.8 kDa (125 amino acids including N-terminal methionine)
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

referenceNicola, N., Nicola, N. Guidebook to Cytokines and Their Receptors NY, NY , (1994), 171
 Kelso, A., Cytokines: principles and prospects. Immunol. Cell Biol. 76, 300, (1998)